IN VIVO IMAGING EQUIPMENTS
In vivo imaging of laboratory animals have emerged as a critical component of preclinical research. Small animal imaging provides a noninvasive means of investigating biological function and structure in vivo. The aim of preclinical molecular imaging studies is the investigation of the biodistribution of specific radiolabeled molecules in experimental animal models using modern dedicated small animal imaging devices, such as nanoScan PET/MRI or nanoScan SPECT/CT. These imaging equipments allows serial assay of animal models of human oncological, cardiovascular and other diseases. These modern small animal multimodality imaging devices are enable the mapping of a PET or SPECT radiotracer distribution in the living organism (mice, rats, etc.). In addition, they can provide detailed information about the anatomical localization (CT, MRI) of functional and metabolic processions.
POSSIBILITIES BY OUR nanoScan PET/MRI AND nanoScan SPECT/CT:
Nowadays the use of immunocompromised (SCID - Severe Combined Immunodeficiency and nude) mice or rats in preclinical studies is spreading. These animals – due to the lack of the immune system – require specific aseptic enclosure conditions.
IVC cage system (Individually Ventilated Cages) provides sterile conditions (air), the right temperature and humidity in keeping immunocompromised animals.
The system consists of a microprocessor controlled central unit (Smart Flow, Techniplast) as well as racks suitable for rat and mouse cages. Cages are individually ventilated to make sure, that a possible infection does not harm the rest of immunocompromised animals. The interior of the cages are considered "A" to their purity.
ANIMAL HOUSE SERVICES
Investigation of the effect of new anticancer drugs on tumor bearing animals by measuring the changes in the tumor volume and body weight.
We place great emphasis on the optimization of the reaction conditions of each radiolabeling. We focus on low precursor consumption in order to preserve.
Our radiochemistry laboratory is focusing on the application of generator isotopes: 68Ga, 44Sc, 99mTc
Cyclotron isotopes (18F, 11C, 44Sc) are available from the Medical Imaging Clinic.
Scintomics GRP synthesis module
Scintomics GRP is a casette based synthesis module, designed for GMP compliant preparation of radiopharmaceuticals for human diagnostic use.
Microfluidic radiolabeling system
The home-built system consists of Rheodyne valves, a double syringe pump and a heated reactor capillary. It is capable of performing fast labeling condition optimization. It is connected to an UPLC system to permit on-line monitoring of the reactions.
GMP compliant production laboratory: class “C” cleanroom with two Comecer synthesis cells and a class “A” dispensing hotcell and isolator for introduction of raw materials
RADIO HPLC-MS SYSTEM
The Waters Acquity UPLC I-class chromatography system is the latest development in separation technology. It is optimized to achieve the highest resolution of analytes is shortes possible times. It consists of a binary pump (1200 bar), an FTN injector, column manager and a PDA detector. Berthold LB513 radioactivity detector is equipped with plastic scintillator cells developed for PET isotopes. It is sensitive and has a wide dynamic range: 10 kBq-10 Bq 68Ga. Xevo G2 Q-TOF mass spectrometer that is used for the identifiaction of new precursors.
Ion source: ESI
Mass analyzer: oaTOF, mass range: 20-100.000 m/z, resolving power >40.000 FWHM.
Mass measurement accuracy: better than 1 ppm
Dynamic range: 4 orders of magnitude
Collision cell: software programmable collision energy control