Radiochemistry

With the facilities of the ScanoMed Radiochemistry Laboratory we can give a helping hand in every step of radiopharmaceutical design from the structure identification to the optimization of the radiolabelling conditions. We are in close collaboration with the University of Debrecen, which provides us countless nuclear medicine and clinical opportunities to serve the very needs of our customers.

Tracer design4U

We design and synthetize chelator-peptide and chelator-protein conjugates according to the needs of our customers. We perform the structural identification in house using mass spectrometry. The product is purified by semipreparative HPLC. The precursors are labelled in small volume with concentrated radiometal solutions to achieve high specific activities and to decrease material consumption.

Isotopes

Our radiochemistry laboratory is focusing on the application of generator isotopes. In the past decade the interest of generator-based PET isotopes was rising, that could be noticed by the widespread use of 68Ge/68Ga generators.

Nowadays, the other field of interest is the so called “theranostic approach”, which means the application of the same tracer, labelled with a PET isotope for diagnosis and a therapeutic isotope for treatment. Scandium has numerous attractive properties, as it has a “theranostic isotope-pair” such as the promising PET isotope 44Sc and the therapeutic isotope 47Sc.   
We have an ITG 68Ge/68Ga isotope generator, an in-house built 44Ti/44Sc generator and 99Mo/99mTc generators. Beyond all of these, our cooperation with the Nuclear Medicine Department provides us the opportunity to work with cyclotron-produced isotopes like 18F, 11C and 44Sc.

Tracers

We keep track of commonly used- and novel radiopharmaceuticals to stand ready to provide up-to-date scientific results. Therefore, we have a wide range of precursors in stock that can be labelled with the isotopes, mentioned above.        
On the other hand, we design and synthetize different chelator-peptide and chelator-protein conjugates according to the needs of our customers. We perform the structural identification, analytical measurements and purification using HPLC-RA-MS system and NMR. The labelling reactions are carried out in small volumes (10-100 ml) with high radioactivity-concentrations to achieve high specific activities and to minimize the precursor consumption.

PET radiopharmaceuticals
(68Ga/44Sc)

Avebetrin

DOTA-NAPamide

DOTA-Nitroimidazole

DOTA-NOC

DOTA-[Pro1,Tyr4]bombesin

DOTA-Substance P

HA-DOTATATE

NODAGA-AMBA

NODAGA-RGD

NODAGA-RGD dimer

Pentixafor

PSMA-HBED

In collaboration with the University of Debrecen

[18F]-Fallypride

 [18F]-FAZA

[18F]-FDG

[18F]-FET

[18F]-FHBG

[18F]-FLT

[18F]-FMISO

[18F]-Lansoprazole

[18F]-NaF

 [18F]-T807

[11C]-Choline

 [11C]-Methionine

[11C]-PIB

SPECT radiopharmaceuticals

99mTc-Pertechnetate ([99mTc]TcO4-)

99mTc-MAG3

99mTc-EC

99mTc-ECD

99mTc-HMPAO

99mTc-MIBI

99mTc-DMSA

99mTc-DTPA

99mTc-Phytate

99mTc-Albumon

99mTc-MDP

99mTc-Tetrofosmin

99mTc-Pyrophosphate

99mTc-EHIDA

99mTc-HYNIC-[D-Phe1,Tyr3-Octreotide]

 

Instruments

Comecer synthesis and dispensing system: We have a class “A” dispensing hotcell, three synthesis cells and an isolator for the introduction of raw materials. The hotcells are located in a class “C” cleanroom that makes possible to work under GMP compliant conditions.

Synthesis module: The Scintomics GRP system is a cassette based synthesis module, designed for GMP compliant preparation of radiopharmaceuticals for human diagnostic use. It supports 68Ga labelling with a wide range of different precursors.

 

Capillary evaporation system: This home-built system is capable of concentrating 68Ga or 44Sc from high volume of volatile solution to small volume (10-100 ul) of buffered solution within a few minutes. It uses a HPLC pump and N2 gas to evaporate the liquid in a heated teflon capillary. Right after the drying step we dissolve the 68Ga or 44Sc from the inner surface of the capillary. With the application of this system we can reach approximately 10-100 GBq/ml activity concentration of 68Ga.

Microfluidic radiolabeling system: The home-built system consists of Rheodyne valves, a double syringe pump and a heated reactor capillary. It is capable of performing fast labeling condition optimization with minimal precursor consumption. It is connected to an UPLC system to permit on-line monitoring of the reactions. 

Radio-HPLC-MS System: The Wates Acquity UPLC I-class chromatography system is the latest development in separation technology. It is optimized to achieve the highest resolution of analytes in the shortest possible runtimes. It consists of a binary pump (1200 bar), an FTN injector, a column manager and a PDA detector. The system is also equipped with a Berthold LB513 radioactivity detector with plastic scintillator cells, developed for PET isotopes. It is sensitive and has a wide dynamic range: 10 kBq-10 Bq 68Ga.

Xevo G2 Q-TOF mass spectrometer that is used for the identification of new precursors. The main parameters of the MS are the following:
- Ion source: ESI
- Mass analyzer: oaTOF, mass range: 20-100.000 m/z, resolving power >40.000 FWHM.
- Mass measurement accuracy: better than 1 ppm
- Dynamic range: 4 orders of magnitude
- Collision cell: software programmable collision energy control

Radio-TLC: The Raytest Gina Star TLC system is well known for reliable and fast radiochemical purity determination.

Gamma counter: The Perkin Elmer Wizard is a low activity counter and gamma spectrometer for ex vivo animal studies and radionuclide purity determination